bcl2 d17c4 antibody  (Cell Signaling Technology Inc)

Journal: Ibrain

Article Title: Cordycepin mediates neuroprotection against apoptosis via ERK/CREB signaling activation in Aβ 1–42 ‐induced neuronal cell models

doi: 10.1002/ibra.12192

Figure Lengend Snippet: Detail information of antibodies.

Article Snippet: Bcl‐2 (D17C4) rabbit mAb , 3498s , CST , Rabbit , 1:1000.

Techniques:

Journal: Pharmaceuticals

Article Title: IL-13Rα2 Is Involved in Resistance to Doxorubicin and Survival of Osteosarcoma Patients

doi: 10.3390/ph17111526

Figure Lengend Snippet: IL-13Rα2 is involved in the regulation of signaling molecules related to osteosarcoma cell proliferation and apoptosis: ( A ) Two osteosarcoma cells, U2OS and KHOS/NP, were transfected with empty vector, shRNA for IL-13Rα2, or wild-type IL-13Rα2 plasmids, and Western blotting performed for IL-13Rα2, TGF-β, snail, cyclin D1, BAX, BCL2, and actin. ( B ) Quantitative reverse-transcription polymerase chain reaction was performed for IL-13Rα2, TGF-β1, snail, cyclin D1, BAX, BCL2, and GAPDH in U2OS and KHOS/NP cells after transfection of vectors. The data were normalized with GAPDH expression. *** p < 0.001; EV, empty vector for wild-type IL-13Rα2; IL-13Rα2-OE, vector for wild-type IL-13Rα2; shControl, control vector for shRNA; shIL-13Rα2, vector for shRNA for IL-13Rα2.

Article Snippet: The following antibodies were used in Western blotting: IL-13RA2/CD213a2 (E7U7B) (#85677, Cell Signaling Technology, Beverly, MA, USA), transforming growth factor β (TGF-β) (#3709, Cell Signaling Technology, Beverly, MA, USA), snail (#ab180714, Abcam, Cambridge, UK), cyclin D1 (#2922, Cell Signaling Technology, Beverly, MA, USA), BAX (#2774, Cell Signaling Technology, Beverly, MA, USA), BCL2 (D17C4) (#3498, Cell Signaling Technology, Beverly, MA, USA), forkhead box O3 (FOXO3a) (75D8) (#2497, Cell Signaling Technology, Beverly, MA, USA), cleaved caspase-3 (Asp175) (5A1E) (#9664, Cell Signaling Technology, Beverly, MA, USA), and actin (#sc-47778, Santa Cruz Biotechnology, Santa Cruz, CA, USA).

Techniques: Transfection, Plasmid Preparation, shRNA, Western Blot, Reverse Transcription, Polymerase Chain Reaction, Expressing, Control

Journal: Pharmaceuticals

Article Title: IL-13Rα2 Is Involved in Resistance to Doxorubicin and Survival of Osteosarcoma Patients

doi: 10.3390/ph17111526

Figure Lengend Snippet: Effects of IL-13Rα2 on apoptosis of osteosarcoma cells under treatment with doxorubicin: ( A ) U2OS and KHOS/NP cells were transfected with vectors, followed by 0.2 μM doxorubicin treatment and Western blot for IL-13Rα2, FOXO3a, BAX, BCL2, cleaved caspase 3, and actin was performed. ( B ) Flow cytometric analysis for apoptosis of osteosarcoma cells under treatment with doxorubicin. U2OS and KHOS/NP cells transfected with vectors were treated with 0.2 μM doxorubicin for 24 h, stained with Annexin V and propidium iodide, and examined using flow cytometry. The right upper and right lower quadrants were considered apoptotic populations. * p < 0.05; *** p < 0.001; ns , not significant; EV, empty vector for wild-type IL-13Rα2; IL-13Rα2-OE, vector for wild-type IL-13Rα2; shControl, control vector for shRNA; shIL-13Rα2, vector for shRNA for IL-13Rα2; DOX, doxorubicin.

Article Snippet: The following antibodies were used in Western blotting: IL-13RA2/CD213a2 (E7U7B) (#85677, Cell Signaling Technology, Beverly, MA, USA), transforming growth factor β (TGF-β) (#3709, Cell Signaling Technology, Beverly, MA, USA), snail (#ab180714, Abcam, Cambridge, UK), cyclin D1 (#2922, Cell Signaling Technology, Beverly, MA, USA), BAX (#2774, Cell Signaling Technology, Beverly, MA, USA), BCL2 (D17C4) (#3498, Cell Signaling Technology, Beverly, MA, USA), forkhead box O3 (FOXO3a) (75D8) (#2497, Cell Signaling Technology, Beverly, MA, USA), cleaved caspase-3 (Asp175) (5A1E) (#9664, Cell Signaling Technology, Beverly, MA, USA), and actin (#sc-47778, Santa Cruz Biotechnology, Santa Cruz, CA, USA).

Techniques: Transfection, Western Blot, Staining, Flow Cytometry, Plasmid Preparation, Control, shRNA

Journal: Pharmaceuticals

Article Title: IL-13Rα2 Is Involved in Resistance to Doxorubicin and Survival of Osteosarcoma Patients

doi: 10.3390/ph17111526

Figure Lengend Snippet: Primer sequences used for a quantitative reverse-transcription polymerase chain reaction.

Article Snippet: The following antibodies were used in Western blotting: IL-13RA2/CD213a2 (E7U7B) (#85677, Cell Signaling Technology, Beverly, MA, USA), transforming growth factor β (TGF-β) (#3709, Cell Signaling Technology, Beverly, MA, USA), snail (#ab180714, Abcam, Cambridge, UK), cyclin D1 (#2922, Cell Signaling Technology, Beverly, MA, USA), BAX (#2774, Cell Signaling Technology, Beverly, MA, USA), BCL2 (D17C4) (#3498, Cell Signaling Technology, Beverly, MA, USA), forkhead box O3 (FOXO3a) (75D8) (#2497, Cell Signaling Technology, Beverly, MA, USA), cleaved caspase-3 (Asp175) (5A1E) (#9664, Cell Signaling Technology, Beverly, MA, USA), and actin (#sc-47778, Santa Cruz Biotechnology, Santa Cruz, CA, USA).

Techniques: Reverse Transcription, Sequencing